Real-Time PCR Detection Kit Porcine Epidemic Diarrhea, Transmissible Gastroenteritis, Porcine Deltacoronavirus Disease Virus and Swine Rotavirus Diagnostic Kit

Product Description

Real-time PCR Detection Kit for Porcine Epidemic Diarrhea, Transmissible Gastroenteritis, Porcine Deltacoronavirus Disease Virus and Swine Rotavirus

[Product Name] Real-time PCR Detection Kit for Porcine Epidemic Diarrhea, Transmissible Gastroenteritis, Porcine Deltacoronavirus Disease Virus and Swine Rotavirus
[Package] 50 Tests
[Indication] The Real-time PCR Detection Kit for Porcine Epidemic Diarrhea, Transmissible Gastroenteritis, Porcine Deltacoronavirus Disease Virus and Rotavirus is applicable for detection, typing, auxiliary diagnosis and epidemiological investigation of the PED/TGEV/PDCoV/RV in porcine serum, plasma, intestinal tissue, intestinal contents, mesenteric lymph nodes, excreta and other samples. The test results are for research purpose only and not for clinical diagnosis.
[Main components and content]

Name	Specification	Quantity
PED/TGEV/PDCoV/RV Reaction Solution	1000µl/Tube	1
PED/TGEV/PDCoV/RV Positive Control	250µl/Tube	1
Negative Control	250µl/Tube	1

[Storage and Shelf Life]
Stored at -20±5ºC, Repeated freezing and thawing ≤ 3 times, the shelf life is 12 months.
[Model]
For ABI QuantStudio 5,CFX Opus 96,LightCycler480,Gentier 96E/96R,LineGene 9600 Plus and other thermal cyclers.
[Test Method]

1.Nucleic Acid Extraction

A commercialized DNA/RNA extraction kit can be carried out for nucleic acid extraction, please follow the instruction of the kit.

2.PCR amplification

2.1 Calculate the number of test samples, take n+2 PCR reaction tubes, and add 20µl to of reaction solution to each tube.
2.2 Add 5µl nucleic acid of negative control, positive control and samples into the above PRC reaction tubes respectively, centrifuge at 8000rpm for several seconds, and put them into the thermal cycler.
2.3 The reaction conditions are set as follows:

Relevant parameters of the amplifier
System	Total volume: 30µl
Signal collection	PED/TGEV/PDCoV/RV quadrivalent fluorescence signal	Transmissible Gastroenteritis (TGEV) - HEX channel collects fluorescence signal
		Porcine Epidemic Diarrhea (PED) - FAM channel collects fluorescence signal
		Porcine Deltacoronavirus Disease Virus (PDCoV) - Cy5 channel collects fluorescence signal
		Swine Rotavirus (RV) - ROX channel collects fluorescence signal
PCR reaction conditions	Stage	Condition	Cycle number
	Reverse Transcription	55ºC: 15 minutes	1
	Predegeneration	95ºC: 30 seconds	1
	PCR	95ºC: 10 seconds	40
		60ºC: 30 seconds (Set to collect fluorescent signal at the end of this stage)

[Interpretation of the results]

1.Determination of test kit effectiveness :

(1)Positive control: Ct value of FAM, HEX, Cy5 and ROX channels ≤ 32, amplification curve with obvious exponential phase.

(2)Negative control: FAM, HEX, Cy5 and ROX channels have no amplification curve, or the amplification curve is straight or slight oblique.

2.Determination of the results:

Result Judgement	FAM channel	HEX channel	Cy5 channel	ROX channel
TGEV acid positive	-	+	-	-
PED nucleic acid positive	+	-	-	-
PDCoV nucleic acid positive	-	-	+	-
RV nucleic acid positive	-	-	-	+
TGEV and PED nucleic acid positive	+	+	-	-
TGEV and PDCoV nucleic acid positive	-	+	+	-
PED and PDCoV nucleic acid positive	+	-	+	-
TGEV and RV nucleic acid positive	-	+	-	+
PED and RV nucleic acid positive	+	-	-	+
PDCoV and RV nucleic acid positive	-	-	+	+
TGEV, PED and RV nucleic acid positive	+	+	-	+
TGEV, PDCoV and RV nucleic acid positive	+	-	+	+
PED, PDCoV and RV nucleic acid positive	-	+	+	+
TGEV, PED and PDCoV nucleic acid positive	+	+	+	-
TGEV, PED, PDCoV and RV nucleic acid positive	+	+	+	+
TGEV, PED, PDCoV and RV nucleic acid negative	-	-	-	-

*Note:
If there is an Exponential growth amplification curve and Ct value ≤ 36, it is determined as "+".
If there is no amplification curve, it is determined as "-".
If 36 < Ct Value < 40, it is a doubtful sample, and the analysis should be repeated for confirmation.
[Precautions]

1. The laboratory management shall strictly follow the management specification of PCR gene amplification laboratory. The laboratory personnel must be trained professionally. The experiment process shall be conducted strictly in different areas (Reagent preparation area, specimen preparation area, amplification and product analysis area). All consumables shall be disposable after sterilization. Special apparatus, equipment and supplies at each stage of the experiment operation shall not be cross used.
2. Please prepare the biological safety cabinet for the reagent and specimen preparation stage. The Lab coat, disposable gloves and pipette shall be carried out during the experiment.
3. Repeated freezing&thawing of reagents shall be avoided as far as possible. Before use, the reagents shall be completely thawed and centrifuged at 8000rpm for several seconds.
4. Please put the pipette used in the specimen preparation area into the container containing disinfectant and discard with the waste after sterilization.
5. After the experiment, the worktable and pipette shall be treated with 10% hypochlorite or 75% alcohol or ultraviolet lamp.

[Manufacture]
Name: Shandong Xinda Gene Technology Co., Ltd
A subsidiary of the Shandong Sinder Technology Co., Ltd
Address: Building B2, Bandaohuigu Industrial Park, Shungeng Road, Zhucheng City, Shandong Province
Post Code: 262233
Phone: +86 - 0532 5882 0810
 

Shandong Sinder Technology Co., Ltd ('Sinder') founded in April 1999, has a wide range of animal health products. Sinder focus on Animal Health Products, Animal Vaccine, Antibodies, Antibiotics, Feed Additives, Chinese Herbal Product, Pet food, Elisa, and PCR test kit.

Sinder always persists in the core concept of 'The Relentless Pursuit of Increasing Customer Value. ' Based on the animal health to achieve a service provider for ecological construction of agriculture and animal husbandry. Support the efficient and sustainable development of agriculture and animal husbandry. Make food safer and rural areas better!

Sinder is a high-tech enterprise and one of the top 10 veterinary drug manufacturers in China and focusing on the animal health business. At the moment, the company has 35 GMP production lines, 1 CNAS laboratory, 3 national scientific research platforms, and 8 provincial scientific research platforms owned by Sinder.

Sinder has 4 R&D centers located in Qingdao, Zhucheng, and Beijing, China, and 1 laboratory in Silicon Valley in the United States. At present, the company has 1,535 employees including more than 200 masters or above and 18 doctors. Also, the company established extensive technical cooperation with several domestic and foreign scientific research institutes, animal health companies, and domestic veterinary medicine enterprises. Sinder has undertaken 57 national, provincial, and ministerial projects, gained 7 provincial and ministerial science and technology progress awards, presided over or participated in 23 national standards, obtained 23 new veterinary drug certificates(one 1st class new drug), and 60 authorized patents.
 

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