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Porcine Circovirus Type 3 (PCV3) Antibody ELISA Test Kit
For Animal Use Only
Product Name: Porcine Circovirus Type 3 (PCV3) Antibody ELISA Test Kit
Main Component and Composition:
|
Name |
Quantity |
|
(1) Coated PCV3 Assay Plate |
96wells x 5 |
|
(2) PCV3 Positive Control Serum (3) PCV3 Negative Control Serum (4) Enzyme Conjugate (5) Wash Buffer (10 x concentrate) (6) Sample Diluent (7) TMB Chromogen Substrate (8) Stop Solution (9) Instruction Manual (10) Reagent Reservoirs (11) Adhesive Strip |
1.5ml x 2 1.5ml x 2 55ml x 1 150ml x 1 100ml x 1 105ml x 1 55ml x 1 1 5 5 |
Sample Preparation:
1. Dilute the sample 150 times with the sample diluent (e.g. add 1μl sample into 149μl sample diluent, mix well).
2. The wash buffer (10 x concentrate) shall be diluted 10 times by deionized water before use. (e.g. add 9ml deionized water to every 1ml wash buffer (10 x concentrate))
3. Negative and positive control serum shall be directly used without dilution.
Usage:
1. Before use, place the kit at room temperature (15-25ºC) for 30min and restore to room temperature.
2. Take the required amount of Coated PCV3 Assay Plate, and set 2 wells for negative and positive controls respectively.
3. Add 100μl positive/negative control to the positive/negative control well, and add 100μl diluted sample to the sample well, incubate at 37ºC for 30min in shading light.
4. Remove the liquid in each well. Immediately add 250μl Wash Buffer to each well and wash. Repeat wash procedure for 3 times, invert the plate and pat it against absorbent paper.
5. Add 100μl of Enzyme Conjugate into each well, cover the plate sealer and incubate at 37ºC for 60min.
6. Remove the liquid in each well. Immediately add 350μl Wash Buffer to each well and wash. Repeat wash procedure for 6 times, invert the plate and pat it against absorbent paper.
7. Add 100µL TMB chromogen Substrate into each well. Cover the plate sealer and mix thoroughly, incubate at 37ºC for 5 min in dark place.
8. Add 50µL Stop Solution in to each well, mix thoroughly. Measure the Absorbance value of each well at 450nm wavelength of Microplate Reader immediately.
Interpretation of the results
1. Validity of the test: the difference between the average OD value of the positive control and negative control > 0.50.
2. The sample OD value is S. The mean value of positive control is Pp, the mean value of negative control is Pn.
3. Calculate: S/P value
4. Judgement: Positive when S/P value ≥ 0.5. Negative when S/P value < 0.5
Cautions:
1. TMB Chromogen Substrate and Stop solution may be irritating to skin and eyes. Please keep away from direct touch and light.
2. The Coated PCV3 Assay Plate should be recovered from cold storage environment to room temperature before opening the package. The unused Coated PCV3 Assay Plate shall be stored in sealed bag with desiccant.
3. The serum shall be collected according to the routine method to avoid hemolysis or bacterial growth. The frozen serum shall avoid repeated freezing and thawing. In case of turbidity or sedimentation, it shall be centrifuged or filtered for clarification before testing.
4. The components of different batch numbers shall be not mixed.
Package: 96T x 5/Box
Storage and Shelf Life: The kit shall be stored at 2-8ºC for 12 months. The unused plate shall be kept in a seal bag at 2-8ºC away from light, the validity will be 1 month.
Shandong Sinder Technology Co., Ltd ('Sinder') founded in April 1999, has a wide range of animal health products. Sinder focus on Animal Health Products, Animal Vaccine, Antibodies, Antibiotics, Feed Additives, Chinese Herbal Product, Pet food, Elisa, and PCR test kit.
Sinder always persists in the core concept of 'The Relentless Pursuit of Increasing Customer Value. ' Based on the animal health to achieve a service provider for ecological construction of agriculture and animal husbandry. Support the efficient and sustainable development of agriculture and animal husbandry. Make food safer and rural areas better!
Sinder is a high-tech enterprise and one of the top 10 veterinary drug manufacturers in China and focusing on the animal health business. At the moment, the company has 35 GMP production lines, 1 CNAS laboratory, 3 national scientific research platforms, and 8 provincial scientific research platforms owned by Sinder.
Sinder has 4 R&D centers located in Qingdao, Zhucheng, and Beijing, China, and 1 laboratory in Silicon Valley in the United States. At present, the company has 1,535 employees including more than 200 masters or above and 18 doctors. Also, the company established extensive technical cooperation with several domestic and foreign scientific research institutes, animal health companies, and domestic veterinary medicine enterprises. Sinder has undertaken 57 national, provincial, and ministerial projects, gained 7 provincial and ministerial science and technology progress awards, presided over or participated in 23 national standards, obtained 23 new veterinary drug certificates(one 1st class new drug), and 60 authorized patents.
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